Technology

Multiplex PCR technology

Multiplicon’s multiplex PCR technology (an algorithm for the design of multiplex PCR primers) and our expertise are based on years of wet-lab experiments that proved the validity of our approach and resulted in a number of applications:

• Multiplex Amplicon Quantification (MAQ)
• Multiplex PCR based 454 sequencing
• Multiplex based microsatellite genotyping

Every application benefits fully from the advantages of multiplex PCR: a large reduction in time and costs compared to standard PCR methods and other molecular approaches.

All of these applications are based on the same principle: primers are designed that enable the simultaneous amplification of several targets in one reaction. When brought to the bench, they work: we performed succesfull multiplex PCR amplification of up to 50 targets.

Due to the robust primer design, we do not encounter the problems generally associated with multiplex PCR like generation of non-specific fragments, lack of amplification product, etc. Moreover, multiplex PCR optimization consists solely of primer concentration adjustments since all primerpairs work under the same conditions.